Abstracts

Share on facebook
Share on twitter
Share on linkedin

Neonatal T Cells Acquire Both Pro-Inflammatory And Tolerant Phenotypes In First Three Weeks Of Life

Authors
Gergely Toldi, Hannah Wood, Richard Powell, Paul Moss, Andrew Ewer

Institution(s)
1 – Department of Neonatology, Birmingham Women’s and Children’s NHS FT
2 – Institute of Immunology and Immunotherapy, University of Birmingham
3 – Institute of Metabolism and Systems Research, University of Birmingham

Introduction
The neonatal immune system needs to rapidly adapt to environmental changes following delivery in order to fight pathogens and tolerate harmless antigens. The precise kinetics of these changes and the key cellular and soluble components of the immune system remain to be elucidated. We aimed to describe changes in the immune phenotype of neonates in the first 3 weeks of life following delivery.

Methods
We collected cord blood samples from 19 healthy, term neonates delivered by elective Caesarean sections. A predelivery maternal peripheral blood sample was also obtained. The neonates were re-sampled for peripheral blood at 3 weeks of age. Mononuclear cells were isolated from these samples and were measured on 2 flow cytometry panels consisting of a total of 20 intracellular and cell surface markers.

Results
The proportion of IL-8-producing CD4 and CD8 cells was lower in maternal compared to neonatal samples. Intracellular IL-17 production of CD4 and CD8 cells increased by 3 weeks of age compared to cord blood. The proportion of Tregs (CD4+ CD25high FoxP3+) increased by 3 weeks compared to cord blood, but remained lower in comparison to maternal samples. The proportion and cytotoxic activity (CD107a+) of CD8 cells, as well as the proportion of IFN-gamma-producing CD4 and CD8 cells were lower in neonatal compared to maternal samples. The proportion of HLA-DR+ and CD69+ T cells was also lower in neonatal compared to maternal samples.

Conclusions
While neonatal T cells maintain an IL-8 phenotype during the first 3 weeks of life, they also acquire an IL-17 phenotype, possibly contributing to their ability to fight infection. On the other hand, the proportion of Tregs also increase by 3 weeks of age, playing an important role in establishing tolerance towards harmless environmental antigens.

More to explorer

Summer Meeting 2024

19th-21st June 2024 Joint Summer Meeting with the Fetal and Neonatal Physiological Society at University of Nottingham Full 3-day, International Conference |

Spring Meeting 2024

14th March 2024 Hybrid event: Virtual meeting or in person at Royal Society of Medicine, London 09.30 – 18.00 GMT Register Here

Autumn Meeting 2023

9th November 2023 Hybrid event: This will be a hybrid meeting with both in-person attendance at the Royal Society of Medicine, London

Search by category

We use cookies to improve your experience on our website. By browsing this website, you agree to our use of cookies.